Lattice Light-Sheet Microscope
This setup is a cutting-edge live cell imaging platform for multi-color high-speed volumetric imaging using thin light-sheets based on optical lattices. It is a clone of the original lattice light-sheet microscope designed in the Eric Betzig group and published in October 2014 (Chen et al., Science). With up to 200 frames per second imaging speed and almost true isotropic resolution, the lattice scope is the fasted 3D imaging system at our facility. Its very thin light-sheets generate highest signal-to-noise ratios and even allow for single molecule imaging throughout the entire cell volume. The latter complements our SMLM expertise using TIRF and HILO imaging.
Highly recommended papers
- Chen, B. C. et al. Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science. 346, 1257998 (2014).
- Li, D. et al. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science. 349, aab3500 (2015).
- Legant, W. R. et al. High-density three-dimensional localization microscopy across large volumes, Nat. Meth. 13, 359-365 (2016).
- Liu, T.-L. et al. Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science. 360, eaaq1392 (2018).
- Gao, R. et al. Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution. Science. 363, eaau8302 (2019).